From Sequencer to ASV Table

After your sequencing run is complete, there are a few methods to obtain the sequencing data in its raw format for downstream analysis. (1) Download from basespace when the run has been linked, or (2) download directly from an external drive that may or may not be connected to the Illumina machine.

A. Download from BaseSpace

In order to download from BaseSpace, the first thing you will need to do is download the Command Line Interface (CLI) for BaseSpace following the directions here https://developer.basespace.illumina.com/docs/content/documentation/cli/cli-overview using the Linux method. You will want to install onto Wynton in your home (~) directory. Specifically run the following commands on Wynton:

cd ~
wget "https://api.bintray.com/content/basespace/BaseSpaceCLI-EarlyAccess-BIN/latest/\$latest/amd64-linux/bs?bt_package=latest" -O $HOME/bin/bs
chmod u+x $HOME/bin/bs
~/bin/bs auth

The output will be a link that you copy into your browser, and you will sign into basespace, thereby linking the bs command to your account. You will only need to do this once. Once complete, return to the command line.

There are several options available once you have BaseSpace installed. Review the link above to find the commands you need. Commands like ~/bin/bs list run will list out all of the runs that are available to you, including the project identifier, which will be helpful when downloading (~/bin/bs download run --id {id} --output {run_name}). Please cp your workspace to the /wynton/group/lynch/NextSeq_data/ directory before running the download command so that the run downloads to our raw run directory.

B. Download From External Drive/Sequencer

If you completed the steps in A and find that within the run directory you don’t have a directory called Data, you may need to download directly from the external drive connected to the sequencer. If connected, the data should be there already, so you can simply walk the drive to your computer.

Now, you will upload the data to Wynton where we will do all sequence data processing.

Start by plugging your drive into your computer. If you have a Mac, there are two Terminal commands that can upload data: scp and rsync. There are benefits to both, but for the purpose of uploading sequence data, scp is better because it gives informative progress text. An example usage for your needs would be:

scp -r ~/Volumes/MyDrive/190826_NS500170_0076_AH772CBGXC/ {username}@dt2.wynton.ucsf.edu:/wynton/group/lynch/NextSeq_data/

A couple of things to note:

The -r option means that it will upload all of the files within the run directory.
Here we are uploading using the data transfer node on Wynton (hence, dt2), which offers much faster upload speeds than using a login node. We are also uploading to a directory on Wynton where our raw runs live. This is important for downstream processing.

If you are running on a Windows machine, you will have more success with software like FileZilla and using an SFTP connection (port 22!). Speak with Katie if you need support with this.

You will now need to upload the mapping file using the same method as above, and it will need to be saved in /wynton/group/lynch/NextSeq_Processed/mapping_files. This will then set you up perfectly for processing the data using the developed script.

Pipeline #1 (QIIME mapping file)

We originally built our sequencing data pipelines to utilize QIIME mapping files. We now use the Illumina-based mapping files for most new runs, but the QIIME mapping file pipeline can be helpful for “legacy” sequencing runs without needing to fully re-build your mapping file. This file has a .txt extension and typically starts with a header of column names that include #SampleID first and Description at the end.

Required Rules for QIIME-based Mapping Files

Mapping files need to be in a very specific format for the software we use to be able to read them in correctly. You can find all of the details here or here. The long-and-short of it is that your mapping file may not pass QC for a number of reasons (one of the main reasons the whole pipeline breaks within the first 10 minutes). The rules are:

The file should be named with the same date as the run (so Nextseq_YYMMDD_mapping.txt matching YYMMDD_Nextseq500_...). PLEASE DO THIS. It matches a run to a mapping file. Three years down the road, we won’t care that you made your mapping file two days after you started the run, we just want to know what run it matches to, and the date is the most interpretable and memorable marker of that.
Should be a tab-delimited file (txt)
The first variable needs to be “#SampleID” (with the hashtag/pound sign, and all applicable capitalization)
Second and third columns should be BarcodeSequence and LinkerPrimerSequence, in that order, though LinkerPrimerSequence contents don’t matter (I believe). Barcode sequence definitely matters.
No duplicate column names
NO BLANK VALUES – ANYWHERE. The new tracking sheet highlights unfilled values in red. Please check that page for any blank values and fill them in before downloading the mapping file as txt.
With QIIME, while periods (.) are okay, you can’t use underscores (_), dashes(-), or any other special characters in the entire file.
We have also discussed adding other descriptive columns to the mapping file including final DNA concentration or 16S copy number, just as additional QC checks if we need to make sure a sample’s composition is consistent with other measured factors from processing
The last column needs to be called “Description” and be filled with the sample name again
All negative controls and empty wells should use “NTC” and “EMTPY” respectively in the SampleType variable. This is the variable used in the Negative Control filtering step to identify negative controls in the dataset. If the script breaks at the negative control filtering step, this is a very likely culprit.

An example of an older mapping file that works with the script, with sample names changed to generic. Clearly you want to make sure that the sample names match up with the data you’re about to analyze. This dummy file can be downloaded from the Lynch Lab Pipeline website.

#SampleID	BarcodeSequence	LinkerPrimerSequence	ForwardPrimer	PrimerPlate	PrimerWell	Sort	Project	WhoExtracted	DNA.Conc	AnySampleRemaining	SampleType	SampleType2	Description
Sample1	TCCCTTGTCTCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A1	1	MUPPITS	Doug	0.7810783	Y	SAMPLE	NASAL	Sample1
Sample2	ACGAGACTGATT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A2	2	MUPPITS	Doug	0.1288942	N	SAMPLE	NASAL	Sample2
Sample3	GCTGTACGGATT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A3	3	MUPPITS	Doug	0.1424257	Y	SAMPLE	NASAL	Sample3
Sample4	ATCACCAGGTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A4	4	MUPPITS	Doug	0.3075904	Y	SAMPLE	NASAL	Sample4
Sample5	TGGTCAACGATA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A5	5	MUPPITS	Doug	0.0332309	Y	SAMPLE	NASAL	Sample5
Sample6	ATCGCACAGTAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A6	6	MUPPITS	Doug	0.1250810	Y	SAMPLE	NASAL	Sample6
Sample7	GTCGTGTAGCCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A7	7	MUPPITS	Doug	0.4200027	Y	SAMPLE	NASAL	Sample7
Sample8	AGCGGAGGTTAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A8	8	MUPPITS	Doug	0.5646221	Y	SAMPLE	NASAL	Sample8
Sample9	ATCCTTTGGTTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A9	9	MUPPITS	Doug	0.1834395	N	SAMPLE	NASAL	Sample9
Sample10	TACAGCGCATAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A10	10	MUPPITS	Doug	0.4065154	Y	SAMPLE	NASAL	Sample10
Sample11	ACCGGTATGTAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A11	11	MUPPITS	Doug	0.0527222	Y	NTC	NTC	Sample11
Sample12	AATTGTGTCGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	A12	12	MUPPITS	Doug	0.3167599	Y	SAMPLE	NASAL	Sample12
Sample13	TGCATACACTGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B1	13	MUPPITS	Doug	0.5117164	Y	SAMPLE	NASAL	Sample13
Sample14	AGTCGAACGAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B2	14	MUPPITS	Doug	0.8168699	Y	SAMPLE	NASAL	Sample14
Sample15	ACCAGTGACTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B3	15	MUPPITS	Doug	0.4636830	Y	SAMPLE	NASAL	Sample15
Sample16	GAATACCAAGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B4	16	MUPPITS	Doug	0.0297815	Y	SAMPLE	NASAL	Sample16
Sample17	GTAGATCGTGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B5	17	MUPPITS	Doug	0.9849059	Y	SAMPLE	NASAL	Sample17
Sample18	TAACGTGTGTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B6	18	MUPPITS	Doug	0.0744560	Y	SAMPLE	NASAL	Sample18
Sample19	CATTATGGCGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B7	19	MUPPITS	Doug	0.0708397	Y	SAMPLE	NASAL	Sample19
Sample20	CCAATACGCCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B8	20	MUPPITS	Doug	0.6100793	Y	SAMPLE	NASAL	Sample20
Sample21	GATCTGCGATCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B9	21	MUPPITS	Doug	0.1023721	Y	SAMPLE	NASAL	Sample21
Sample22	CAGCTCATCAGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B10	22	MUPPITS	Doug	0.7359677	Y	SAMPLE	NASAL	Sample22
Sample23	CAAACAACAGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B11	23	MUPPITS	Doug	0.6867992	Y	SAMPLE	NASAL	Sample23
Sample24	GCAACACCATCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	B12	24	MUPPITS	Doug	0.0001598	Y	SAMPLE	NASAL	Sample24
Sample25	GCGATATATCGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C1	25	MUPPITS	Doug	0.5374159	Y	SAMPLE	NASAL	Sample25
Sample26	CGAGCAATCCTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C2	26	MUPPITS	Doug	0.9209530	Y	SAMPLE	NASAL	Sample26
Sample27	AGTCGTGCACAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C3	27	MUPPITS	Doug	0.3991068	Y	SAMPLE	NASAL	Sample27
Sample28	GTATCTGCGCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C4	28	MUPPITS	Doug	0.1644655	Y	SAMPLE	NASAL	Sample28
Sample29	CGAGGGAAAGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C5	29	MUPPITS	Doug	0.5527741	Y	SAMPLE	NASAL	Sample29
Sample30	CAAATTCGGGAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C6	30	MUPPITS	Doug	0.0183445	Y	NTC	NTC	Sample30
Sample31	AGATTGACCAAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C7	31	MUPPITS	Doug	0.4388797	Y	SAMPLE	NASAL	Sample31
Sample32	AGTTACGAGCTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C8	32	MUPPITS	Doug	0.7926006	Y	SAMPLE	NASAL	Sample32
Sample33	GCATATGCACTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C9	33	MUPPITS	Doug	0.5974992	Y	SAMPLE	NASAL	Sample33
Sample34	CAACTCCCGTGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C10	34	MUPPITS	Doug	0.4818109	Y	SAMPLE	NASAL	Sample34
Sample35	TTGCGTTAGCAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C11	35	MUPPITS	Doug	0.6339705	Y	SAMPLE	NASAL	Sample35
Sample36	TACGAGCCCTAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	C12	36	MUPPITS	Doug	0.6212989	Y	SAMPLE	NASAL	Sample36
Sample37	CACTACGCTAGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D1	37	MUPPITS	Doug	0.0942493	Y	SAMPLE	NASAL	Sample37
Sample38	TGCAGTCCTCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D2	38	MUPPITS	Doug	0.2939857	Y	SAMPLE	NASAL	Sample38
Sample39	ACCATAGCTCCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D3	39	MUPPITS	Doug	0.0326635	Y	SAMPLE	NASAL	Sample39
Sample40	TCGACATCTCTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D4	40	MUPPITS	Doug	0.3041807	Y	SAMPLE	NASAL	Sample40
Sample41	GAACACTTTGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D5	41	MUPPITS	Doug	0.7169907	Y	SAMPLE	NASAL	Sample41
Sample42	GAGCCATCTGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D6	42	MUPPITS	Doug	0.9687156	Y	SAMPLE	NASAL	Sample42
Sample43	TTGGGTACACGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D7	43	MUPPITS	Doug	0.6884949	Y	SAMPLE	NASAL	Sample43
Sample44	AAGGCGCTCCTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D8	44	MUPPITS	Doug	0.7190858	Y	SAMPLE	NASAL	Sample44
Sample45	TAATACGGATCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D9	45	MUPPITS	Doug	0.3639897	Y	SAMPLE	NASAL	Sample45
Sample46	TCGGAATTAGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D10	46	MUPPITS	Doug	0.5806169	Y	SAMPLE	NASAL	Sample46
Sample47	TGTGAATTCGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D11	47	MUPPITS	Doug	0.7083583	Y	SAMPLE	NASAL	Sample47
Sample48	CATTCGTGGCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	D12	48	MUPPITS	Doug	0.0281156	Y	SAMPLE	NASAL	Sample48
Sample49	TACTACGTGGCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E1	49	MUPPITS	Doug	0.6354233	Y	SAMPLE	NASAL	Sample49
Sample50	GGCCAGTTCCTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E2	50	MUPPITS	Doug	0.4567869	Y	SAMPLE	NASAL	Sample50
Sample51	GATGTTCGCTAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E3	51	MUPPITS	Doug	0.9392233	Y	NTC	NTC	Sample51
Sample52	CTATCTCCTGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E4	52	MUPPITS	Doug	0.0834425	Y	SAMPLE	NASAL	Sample52
Sample53	ACTCACAGGAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E5	53	MUPPITS	Doug	0.3666240	Y	SAMPLE	NASAL	Sample53
Sample54	ATGATGAGCCTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E6	54	MUPPITS	Doug	0.1962734	Y	SAMPLE	NASAL	Sample54
Sample55	GTCGACAGAGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E7	55	MUPPITS	Doug	0.6166212	Y	SAMPLE	NASAL	Sample55
Sample56	TGTCGCAAATAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E8	56	MUPPITS	Doug	0.5765813	Y	SAMPLE	NASAL	Sample56
Sample57	CATCCCTCTACT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E9	57	MUPPITS	Doug	0.9462517	Y	SAMPLE	NASAL	Sample57
Sample58	TATACCGCTGCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E10	58	MUPPITS	Doug	0.1937192	Y	SAMPLE	NASAL	Sample58
Sample59	AGTTGAGGCATT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E11	59	MUPPITS	Doug	0.5259539	Y	SAMPLE	NASAL	Sample59
Sample60	ACAATAGACACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	E12	60	MUPPITS	Doug	0.2531618	Y	SAMPLE	NASAL	Sample60
Sample61	CGGTCAATTGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F1	61	MUPPITS	Doug	0.8812484	Y	SAMPLE	NASAL	Sample61
Sample62	GTGGAGTCTCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F2	62	MUPPITS	Doug	0.7694885	Y	SAMPLE	NASAL	Sample62
Sample63	GCTCGAAGATTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F3	63	MUPPITS	Doug	0.4580986	Y	SAMPLE	NASAL	Sample63
Sample64	AGGCTTACGTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F4	64	MUPPITS	Doug	0.5351421	Y	SAMPLE	NASAL	Sample64
Sample65	TCTCTACCACTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F5	65	MUPPITS	Doug	0.1557944	Y	SAMPLE	NASAL	Sample65
Sample66	ACTTCCAACTTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F6	66	MUPPITS	Doug	0.8917240	Y	SAMPLE	NASAL	Sample66
Sample67	CTCACCTAGGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F7	67	MUPPITS	Doug	0.1810661	Y	SAMPLE	NASAL	Sample67
Sample68	GTGTTGTCGTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F8	68	MUPPITS	Doug	0.7592205	Y	SAMPLE	NASAL	Sample68
Sample69	CCACAGATCGAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F9	69	MUPPITS	Doug	0.0034810	Y	SAMPLE	NASAL	Sample69
Sample70	TATCGACACAAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F10	70	MUPPITS	Doug	0.4569359	Y	SAMPLE	NASAL	Sample70
Sample71	GATTCCGGCTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F11	71	MUPPITS	Doug	0.0865273	Y	SAMPLE	NASAL	Sample71
Sample72	CGTAATTGCCGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	F12	72	MUPPITS	Doug	0.0525078	Y	SAMPLE	NASAL	Sample72
Sample73	GGTGACTAGTTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G1	73	MUPPITS	Doug	0.0756458	Y	SAMPLE	NASAL	Sample73
Sample74	ATGGGTTCCGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G2	74	MUPPITS	Doug	0.0727405	Y	SAMPLE	NASAL	Sample74
Sample75	TAGGCATGCTTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G3	75	MUPPITS	Doug	0.9850628	Y	SAMPLE	NASAL	Sample75
Sample76	AACTAGTTCAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G4	76	MUPPITS	Doug	0.8176845	Y	SAMPLE	NASAL	Sample76
Sample77	ATTCTGCCGAAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G5	77	MUPPITS	Doug	0.2321371	Y	SAMPLE	NASAL	Sample77
Sample78	AGCATGTCCCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G6	78	MUPPITS	Doug	0.0944784	Y	SAMPLE	NASAL	Sample78
Sample79	GTACGATATGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G7	79	MUPPITS	Doug	0.3069405	Y	SAMPLE	NASAL	Sample79
Sample80	GTGGTGGTTTCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G8	80	MUPPITS	Doug	0.0043399	Y	SAMPLE	NASAL	Sample80
Sample81	TAGTATGCGCAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G9	81	MUPPITS	Doug	0.5892653	Y	SAMPLE	NASAL	Sample81
Sample82	TGCGCTGAATGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G10	82	MUPPITS	Doug	0.8251904	Y	SAMPLE	NASAL	Sample82
Sample83	ATGGCTGTCAGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G11	83	MUPPITS	Doug	0.0274719	Y	SAMPLE	NASAL	Sample83
Sample84	GTTCTCTTCTCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	G12	84	MUPPITS	Doug	0.3039694	Y	SAMPLE	NASAL	Sample84
Sample85	CGTAAGATGCCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H1	85	MUPPITS	Doug	0.4381283	Y	SAMPLE	NASAL	Sample85
Sample86	GCGTTCTAGCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H2	86	MUPPITS	Doug	0.2787986	Y	SAMPLE	NASAL	Sample86
Sample87	GTTGTTCTGGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H3	87	MUPPITS	Doug	0.8775337	Y	SAMPLE	NASAL	Sample87
Sample88	GGACTTCCAGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H4	88	MUPPITS	Doug	0.5130315	Y	SAMPLE	NASAL	Sample88
Sample89	CTCACAACCGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H5	89	MUPPITS	Doug	0.9927105	Y	SAMPLE	NASAL	Sample89
Sample90	CTGCTATTCCTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H6	90	MUPPITS	Doug	0.1128763	Y	SAMPLE	NASAL	Sample90
Sample91	ATGTCACCGCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H7	91	MUPPITS	Doug	0.3761073	Y	NTC	NTC	Sample91
Sample92	TGTAACGCCGAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H8	92	MUPPITS	Doug	0.5943543	Y	SAMPLE	NASAL	Sample92
Sample93	AGCAGAACATCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H9	93	MUPPITS	Doug	0.4205088	Y	SAMPLE	NASAL	Sample93
Sample94	TGGAGTAGGTGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H10	94	MUPPITS	Doug	0.2136384	Y	SAMPLE	NASAL	Sample94
Sample95	TTGGCTCTATTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H11	95	MUPPITS	Doug	0.9339919	Y	SAMPLE	NASAL	Sample95
Sample96	GATCCCACGTAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE1	H12	96	MUPPITS	Doug	0.4778150	Y	SAMPLE	NASAL	Sample96
Sample97	TCTGAGGTTGCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A1	97	MUPPITS	Doug	0.6961659	Y	SAMPLE	NASAL	Sample97
Sample98	CGATGTGTGGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A2	98	MUPPITS	Doug	0.7906773	Y	NTC	NTC	Sample98
Sample99	TCGTGGATAGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A3	99	MUPPITS	Doug	0.4974306	Y	SAMPLE	NASAL	Sample99
Sample100	ATAATTGCCGAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A4	100	MUPPITS	Doug	0.8925467	Y	SAMPLE	NASAL	Sample100
Sample101	GTACTGAAGATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A5	101	MUPPITS	Doug	0.8376468	Y	SAMPLE	NASAL	Sample101
Sample102	ACTTTGCTTTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A6	102	MUPPITS	Doug	0.1078415	Y	SAMPLE	NASAL	Sample102
Sample103	ATAGGCTGTAGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A7	103	MUPPITS	Doug	0.3242043	Y	SAMPLE	NASAL	Sample103
Sample104	TCAGTCAGATGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A8	104	MUPPITS	Doug	0.1378635	Y	SAMPLE	NASAL	Sample104
Sample105	CTCTATTCCACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A9	105	MUPPITS	Doug	0.5843154	Y	SAMPLE	NASAL	Sample105
Sample106	AACCGATGTACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A10	106	MUPPITS	Doug	0.2096100	Y	SAMPLE	NASAL	Sample106
Sample107	CATGTGCTTAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A11	107	MUPPITS	Doug	0.1358358	Y	SAMPLE	NASAL	Sample107
Sample108	TCCGTGGTATAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	A12	108	MUPPITS	Doug	0.7407449	Y	SAMPLE	NASAL	Sample108
Sample109	GATCATTCTCTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B1	109	MUPPITS	Doug	0.8161994	Y	SAMPLE	NASAL	Sample109
Sample110	GCGAAGTTGGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B2	110	MUPPITS	Doug	0.7508248	Y	SAMPLE	NASAL	Sample110
Sample111	GACGCACTAACT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B3	111	MUPPITS	Doug	0.8949682	Y	SAMPLE	NASAL	Sample111
Sample112	GGCATGTTATCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B4	112	MUPPITS	Doug	0.8589889	Y	SAMPLE	NASAL	Sample112
Sample113	TCGCCGTGTACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B5	113	MUPPITS	Doug	0.8590253	Y	SAMPLE	NASAL	Sample113
Sample114	CAAAGCGGTATT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B6	114	MUPPITS	Doug	0.6608787	Y	SAMPLE	NASAL	Sample114
Sample115	TGTGTAGCCATG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B7	115	MUPPITS	Doug	0.8712229	Y	SAMPLE	NASAL	Sample115
Sample116	AAGTCACACACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B8	116	MUPPITS	Doug	0.9275056	Y	SAMPLE	NASAL	Sample116
Sample117	ATTGAGTGAGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B9	117	MUPPITS	Doug	0.8605978	Y	SAMPLE	NASAL	Sample117
Sample118	TCGATTGGCCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B10	118	MUPPITS	Doug	0.3087016	Y	SAMPLE	NASAL	Sample118
Sample119	GTTCCTCCATTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B11	119	MUPPITS	Doug	0.3072570	Y	SAMPLE	NASAL	Sample119
Sample120	TCTACGGCACGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	B12	120	MUPPITS	Doug	0.1148951	Y	SAMPLE	NASAL	Sample120
Sample121	AGACATACCGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C1	121	MUPPITS	Doug	0.0286788	Y	SAMPLE	NASAL	Sample121
Sample122	GCATTCGGCGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C2	122	MUPPITS	Doug	0.6409927	Y	SAMPLE	NASAL	Sample122
Sample123	GGCGATTTACGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C3	123	MUPPITS	Doug	0.3500111	Y	SAMPLE	NASAL	Sample123
Sample124	AGGCACAGTAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C4	124	MUPPITS	Doug	0.6183724	Y	SAMPLE	NASAL	Sample124
Sample125	AACTGCGATATG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C5	125	MUPPITS	Doug	0.3074030	Y	SAMPLE	NASAL	Sample125
Sample126	CGAAACTACGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C6	126	MUPPITS	Doug	0.0877470	Y	SAMPLE	NASAL	Sample126
Sample127	AAGGGCGCTGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C7	127	MUPPITS	Doug	0.1820293	Y	SAMPLE	NASAL	Sample127
Sample128	GCTGTGATTCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C8	128	MUPPITS	Doug	0.5230592	Y	SAMPLE	NASAL	Sample128
Sample129	TTATGGTACGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C9	129	MUPPITS	Doug	0.6801442	Y	SAMPLE	NASAL	Sample129
Sample130	GCATTACTGGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C10	130	MUPPITS	Doug	0.6394840	Y	SAMPLE	NASAL	Sample130
Sample131	ACCTGTCCTTTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C11	131	MUPPITS	Doug	0.8125320	Y	SAMPLE	NASAL	Sample131
Sample132	ATGCTGCAACAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	C12	132	MUPPITS	Doug	0.7219349	Y	SAMPLE	NASAL	Sample132
Sample133	GATCCTCATGCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D1	133	MUPPITS	Doug	0.5004426	Y	SAMPLE	NASAL	Sample133
Sample134	CGCCATTGTGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D2	134	MUPPITS	Doug	0.6461489	Y	SAMPLE	NASAL	Sample134
Sample135	TAAGGCATCGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D3	135	MUPPITS	Doug	0.1943545	Y	SAMPLE	NASAL	Sample135
Sample136	CTACTTACATCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D4	136	MUPPITS	Doug	0.6574181	Y	SAMPLE	NASAL	Sample136
Sample137	CTTCCAACTCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D5	137	MUPPITS	Doug	0.6014949	Y	SAMPLE	NASAL	Sample137
Sample138	GAGGACCAGCAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D6	138	MUPPITS	Doug	0.3616203	Y	SAMPLE	NASAL	Sample138
Sample139	GTTTCCGTGGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D7	139	MUPPITS	Doug	0.7243769	Y	SAMPLE	NASAL	Sample139
Sample140	CTAGCTATGGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D8	140	MUPPITS	Doug	0.7690715	Y	SAMPLE	NASAL	Sample140
Sample141	GCTAGTTATGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D9	141	MUPPITS	Doug	0.5128853	Y	SAMPLE	NASAL	Sample141
Sample142	TTGGGCCACATA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D10	142	MUPPITS	Doug	0.6086485	Y	NTC	NTC	Sample142
Sample143	GTTCACGCCCAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D11	143	MUPPITS	Doug	0.2842746	Y	SAMPLE	NASAL	Sample143
Sample144	TTCTCATGGAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	D12	144	MUPPITS	Doug	0.7459655	Y	SAMPLE	NASAL	Sample144
Sample145	ATTATCGTCCCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E1	145	MUPPITS	Doug	0.1481712	Y	SAMPLE	NASAL	Sample145
Sample146	TCCAACTGCAGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E2	146	MUPPITS	Doug	0.4849201	Y	SAMPLE	NASAL	Sample146
Sample147	ACCCATACAGCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E3	147	MUPPITS	Doug	0.2934432	Y	SAMPLE	NASAL	Sample147
Sample148	CTCTTCTGATCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E4	148	MUPPITS	Doug	0.0644110	Y	SAMPLE	NASAL	Sample148
Sample149	GAGATCGCCTAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E5	149	MUPPITS	Doug	0.4897648	Y	SAMPLE	NASAL	Sample149
Sample150	AATAGCATGTCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E6	150	MUPPITS	Doug	0.1541524	Y	SAMPLE	NASAL	Sample150
Sample151	AGGAACCAGACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E7	151	MUPPITS	Doug	0.5511208	Y	SAMPLE	NASAL	Sample151
Sample152	CTTGACGAGGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E8	152	MUPPITS	Doug	0.5074784	Y	SAMPLE	NASAL	Sample152
Sample153	CAGATTAACCAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E9	153	MUPPITS	Doug	0.8526273	Y	SAMPLE	NASAL	Sample153
Sample154	CACACAAAGTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E10	154	MUPPITS	Doug	0.3233420	Y	SAMPLE	NASAL	Sample154
Sample155	CGATCGAACACT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E11	155	MUPPITS	Doug	0.8309989	Y	SAMPLE	NASAL	Sample155
Sample156	CATAGTGATTGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	E12	156	MUPPITS	Doug	0.1880192	Y	SAMPLE	NASAL	Sample156
Sample157	CCAGACCGCTAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F1	157	MUPPITS	Doug	0.4818939	Y	SAMPLE	NASAL	Sample157
Sample158	TAAAGACCCGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F2	158	MUPPITS	Doug	0.7410113	Y	SAMPLE	NASAL	Sample158
Sample159	CGCACTACGCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F3	159	MUPPITS	Doug	0.8735439	Y	NTC	NTC	Sample159
Sample160	ATGCTAACCACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F4	160	MUPPITS	Doug	0.7660363	Y	SAMPLE	NASAL	Sample160
Sample161	TGTACATCGCCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F5	161	MUPPITS	Doug	0.0129860	Y	SAMPLE	NASAL	Sample161
Sample162	CGGAGTAATCCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F6	162	MUPPITS	Doug	0.9998649	Y	SAMPLE	NASAL	Sample162
Sample163	TAATGCCCAGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F7	163	MUPPITS	Doug	0.1957232	Y	SAMPLE	NASAL	Sample163
Sample164	ACCTGGGAATAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F8	164	MUPPITS	Doug	0.6361749	Y	SAMPLE	NASAL	Sample164
Sample165	GGCTGCATACTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F9	165	MUPPITS	Doug	0.9655463	Y	SAMPLE	NASAL	Sample165
Sample166	GCCAAGGATAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F10	166	MUPPITS	Doug	0.5835950	Y	SAMPLE	NASAL	Sample166
Sample167	CATGCCAACATG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F11	167	MUPPITS	Doug	0.3507827	Y	SAMPLE	NASAL	Sample167
Sample168	GCTATCAAGACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	F12	168	MUPPITS	Doug	0.8181771	Y	SAMPLE	NASAL	Sample168
Sample169	AGCTCTAGAAAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G1	169	MUPPITS	Doug	0.5626855	Y	SAMPLE	NASAL	Sample169
Sample170	TGTATCTTCACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G2	170	MUPPITS	Doug	0.8336965	Y	SAMPLE	NASAL	Sample170
Sample171	CAGTCGTTAAGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G3	171	MUPPITS	Doug	0.4434997	Y	SAMPLE	NASAL	Sample171
Sample172	ACCAATCTCGGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G4	172	MUPPITS	Doug	0.7606154	Y	SAMPLE	NASAL	Sample172
Sample173	TGTTAAGCAGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G5	173	MUPPITS	Doug	0.1773047	Y	SAMPLE	NASAL	Sample173
Sample174	CTGTGTCCATGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G6	174	MUPPITS	Doug	0.5607500	Y	SAMPLE	NASAL	Sample174
Sample175	TATGAACGTCCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G7	175	MUPPITS	Doug	0.9573854	Y	SAMPLE	NASAL	Sample175
Sample176	CTCTGCCTAATT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G8	176	MUPPITS	Doug	0.7977146	Y	SAMPLE	NASAL	Sample176
Sample177	TTGGTAAAGTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G9	177	MUPPITS	Doug	0.6886710	Y	SAMPLE	NASAL	Sample177
Sample178	CGCCACGTGTAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G10	178	MUPPITS	Doug	0.0876809	Y	SAMPLE	NASAL	Sample178
Sample179	GAGTACAGTCTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G11	179	MUPPITS	Doug	0.9934803	Y	SAMPLE	NASAL	Sample179
Sample180	CCGTGACAACTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	G12	180	MUPPITS	Doug	0.1531490	Y	SAMPLE	NASAL	Sample180
Sample181	TCCATCGACGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H1	181	MUPPITS	Doug	0.7741995	Y	SAMPLE	NASAL	Sample181
Sample182	GACTGACTCGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H2	182	MUPPITS	Doug	0.8576535	Y	SAMPLE	NASAL	Sample182
Sample183	CTACGAAAGCCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H3	183	MUPPITS	Doug	0.1880942	Y	SAMPLE	NASAL	Sample183
Sample184	TATCCAAGCGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H4	184	MUPPITS	Doug	0.5928764	N	SAMPLE	NASAL	Sample184
Sample185	ACGGCGTTATGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H5	185	MUPPITS	Doug	0.4276007	Y	SAMPLE	NASAL	Sample185
Sample186	CTTCGCGGATGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H6	186	MUPPITS	Doug	0.1251030	Y	SAMPLE	NASAL	Sample186
Sample187	CCACATTGGGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H7	187	MUPPITS	Doug	0.8368513	Y	SAMPLE	NASAL	Sample187
Sample188	ATATGACCCAGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H8	188	MUPPITS	Doug	0.2369587	Y	SAMPLE	NASAL	Sample188
Sample189	AAGTGGCTATCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H9	189	MUPPITS	Doug	0.9253431	Y	SAMPLE	NASAL	Sample189
Sample190	GCAACCGATTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H10	190	MUPPITS	Doug	0.8870480	Y	NTC	NTC	Sample190
Sample191	CCTACATGAGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H11	191	MUPPITS	Doug	0.8843871	Y	SAMPLE	NASAL	Sample191
Sample192	CGTTCCTTGTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE2	H12	192	MUPPITS	Doug	0.6778118	Y	SAMPLE	NASAL	Sample192
Sample193	GGAATTATCGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A1	193	MUPPITS	Doug	0.0965138	Y	SAMPLE	NASAL	Sample193
Sample194	CATCAAGCATAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A2	194	MUPPITS	Doug	0.9758595	Y	SAMPLE	NASAL	Sample194
Sample195	CATCGCGTTGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A3	195	MUPPITS	Doug	0.6373252	Y	SAMPLE	NASAL	Sample195
Sample196	GCACATAGTCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A4	196	MUPPITS	Doug	0.1026851	Y	SAMPLE	NASAL	Sample196
Sample197	GGCAAATACACT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A5	197	MUPPITS	Doug	0.4708254	Y	SAMPLE	NASAL	Sample197
Sample198	GTCATGCTCCAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A6	198	MUPPITS	Doug	0.6362806	Y	SAMPLE	NASAL	Sample198
Sample199	CCTAGTAAGCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A7	199	MUPPITS	Doug	0.0504609	Y	SAMPLE	NASAL	Sample199
Sample200	TTACCGACGAGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A8	200	MUPPITS	Doug	0.1310128	Y	NTC	NTC	Sample200
Sample201	GCTTAGATGTAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A9	201	MUPPITS	Doug	0.2389436	Y	SAMPLE	NASAL	Sample201
Sample202	AAGACGTAGCGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A10	202	MUPPITS	Doug	0.4051023	Y	SAMPLE	NASAL	Sample202
Sample203	TTACCTTACACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A11	203	MUPPITS	Doug	0.4221389	Y	SAMPLE	NASAL	Sample203
Sample204	TGACTAATGGCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	A12	204	MUPPITS	Doug	0.6443750	Y	SAMPLE	NASAL	Sample204
Sample205	CTCTCTCACTTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B1	205	MUPPITS	Doug	0.6485542	Y	SAMPLE	NASAL	Sample205
Sample206	ATTGCAAGCAAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B2	206	MUPPITS	Doug	0.4314353	Y	SAMPLE	NASAL	Sample206
Sample207	CACGTGACATGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B3	207	MUPPITS	Doug	0.7572075	Y	SAMPLE	NASAL	Sample207
Sample208	CACAGTTGAAGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B4	208	MUPPITS	Doug	0.9185859	Y	SAMPLE	NASAL	Sample208
Sample209	CTAGGATCACTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B5	209	MUPPITS	Doug	0.7147503	Y	SAMPLE	NASAL	Sample209
Sample210	GATGACCCAAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B6	210	MUPPITS	Doug	0.4578507	Y	SAMPLE	NASAL	Sample210
Sample211	ACCGGAGTAGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B7	211	MUPPITS	Doug	0.7766815	Y	SAMPLE	NASAL	Sample211
Sample212	TGAGGACTACCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B8	212	MUPPITS	Doug	0.6423767	Y	SAMPLE	NASAL	Sample212
Sample213	CAATCGGCTTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B9	213	MUPPITS	Doug	0.8706919	Y	SAMPLE	NASAL	Sample213
Sample214	AACACTCGATCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B10	214	MUPPITS	Doug	0.5193472	Y	SAMPLE	NASAL	Sample214
Sample215	TGACCGGCTGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B11	215	MUPPITS	Doug	0.8207275	Y	SAMPLE	NASAL	Sample215
Sample216	GGAGGAGCAATA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	B12	216	MUPPITS	Doug	0.7480889	Y	SAMPLE	NASAL	Sample216
Sample217	AGCGACGAAGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C1	217	MUPPITS	Doug	0.4300942	Y	SAMPLE	NASAL	Sample217
Sample218	CTTCCCTAACTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C2	218	MUPPITS	Doug	0.0390112	Y	SAMPLE	NASAL	Sample218
Sample219	TGGAAGAACGGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C3	219	MUPPITS	Doug	0.6257586	Y	SAMPLE	NASAL	Sample219
Sample220	GCTAGACACTAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C4	220	MUPPITS	Doug	0.7875898	Y	SAMPLE	NASAL	Sample220
Sample221	TTGGATTGAACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C5	221	MUPPITS	Doug	0.7918226	Y	SAMPLE	NASAL	Sample221
Sample222	GATATACCAGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C6	222	MUPPITS	Doug	0.0626436	Y	NTC	NTC	Sample222
Sample223	AACAAACTGCCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C7	223	MUPPITS	Doug	0.7614896	Y	SAMPLE	NASAL	Sample223
Sample224	GTAGACATGTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C8	224	MUPPITS	Doug	0.8523114	Y	SAMPLE	NASAL	Sample224
Sample225	TACAGTTACGCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C9	225	MUPPITS	Doug	0.8021645	Y	SAMPLE	NASAL	Sample225
Sample226	CAAGCCCTAGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C10	226	MUPPITS	Doug	0.3186841	Y	SAMPLE	NASAL	Sample226
Sample227	TAGTGTCGGATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C11	227	MUPPITS	Doug	0.5832944	Y	SAMPLE	NASAL	Sample227
Sample228	CTGAGCTCTGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	C12	228	MUPPITS	Doug	0.7819756	Y	SAMPLE	NASAL	Sample228
Sample229	CTTCGACTTTCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D1	229	MUPPITS	Doug	0.8941924	Y	SAMPLE	NASAL	Sample229
Sample230	GTCATAAGAACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D2	230	MUPPITS	Doug	0.2893519	Y	SAMPLE	NASAL	Sample230
Sample231	GTCCGCAAGTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D3	231	MUPPITS	Doug	0.6138171	Y	SAMPLE	NASAL	Sample231
Sample232	CGTAGAGCTCTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D4	232	MUPPITS	Doug	0.7523195	Y	SAMPLE	NASAL	Sample232
Sample233	CCTCTGAGAGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D5	233	MUPPITS	Doug	0.8398925	Y	SAMPLE	NASAL	Sample233
Sample234	CCTCGATGCAGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D6	234	MUPPITS	Doug	0.3336248	Y	SAMPLE	NASAL	Sample234
Sample235	GCGGACTATTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D7	235	MUPPITS	Doug	0.7186200	Y	SAMPLE	NASAL	Sample235
Sample236	CGTGCACAATTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D8	236	MUPPITS	Doug	0.9532292	Y	SAMPLE	NASAL	Sample236
Sample237	CGGCCTAAGTTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D9	237	MUPPITS	Doug	0.1212764	Y	SAMPLE	NASAL	Sample237
Sample238	AGCGCTCACATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D10	238	MUPPITS	Doug	0.8152840	Y	SAMPLE	NASAL	Sample238
Sample239	TGGTTATGGCAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D11	239	MUPPITS	Doug	0.6521641	Y	SAMPLE	NASAL	Sample239
Sample240	CGAGGTTCTGAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	D12	240	MUPPITS	Doug	0.6027521	Y	SAMPLE	NASAL	Sample240
Sample241	AACTCCTGTGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E1	241	MUPPITS	Doug	0.2239951	Y	SAMPLE	NASAL	Sample241
Sample242	TAATGGTCGTAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E2	242	MUPPITS	Doug	0.4337306	Y	SAMPLE	NASAL	Sample242
Sample243	TTGCACCGTCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E3	243	MUPPITS	Doug	0.5165084	Y	SAMPLE	NASAL	Sample243
Sample244	TGCTACAGACGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E4	244	MUPPITS	Doug	0.3756992	Y	SAMPLE	NASAL	Sample244
Sample245	ATGGCCTGACTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E5	245	MUPPITS	Doug	0.3781129	Y	SAMPLE	NASAL	Sample245
Sample246	ACGCACATACAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E6	246	MUPPITS	Doug	0.4214507	Y	SAMPLE	NASAL	Sample246
Sample247	TGAGTGGTCTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E7	247	MUPPITS	Doug	0.3069091	Y	SAMPLE	NASAL	Sample247
Sample248	GATAGCACTCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E8	248	MUPPITS	Doug	0.2023576	Y	SAMPLE	NASAL	Sample248
Sample249	TAGCGCGAACTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E9	249	MUPPITS	Doug	0.3668140	Y	SAMPLE	NASAL	Sample249
Sample250	CATACACGCACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E10	250	MUPPITS	Doug	0.4584611	Y	NTC	NTC	Sample250
Sample251	ACCTCAGTCAAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E11	251	MUPPITS	Doug	0.3646465	Y	SAMPLE	NASAL	Sample251
Sample252	TCGACCAAACAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	E12	252	MUPPITS	Doug	0.3964287	Y	SAMPLE	NASAL	Sample252
Sample253	CCACCCAGTAAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F1	253	MUPPITS	Doug	0.4478209	Y	SAMPLE	NASAL	Sample253
Sample254	ATATCGCGATGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F2	254	MUPPITS	Doug	0.5343944	Y	SAMPLE	NASAL	Sample254
Sample255	CGCCGGTAATCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F3	255	MUPPITS	Doug	0.0603492	Y	SAMPLE	NASAL	Sample255
Sample256	CCGATGCCTTGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F4	256	MUPPITS	Doug	0.1923349	Y	SAMPLE	NASAL	Sample256
Sample257	AGCAGGCACGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F5	257	MUPPITS	Doug	0.7871509	Y	SAMPLE	NASAL	Sample257
Sample258	TACGCAGCACTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F6	258	MUPPITS	Doug	0.1855539	Y	SAMPLE	NASAL	Sample258
Sample259	CGCTTAGTGCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F7	259	MUPPITS	Doug	0.3244295	Y	SAMPLE	NASAL	Sample259
Sample260	CAAAGTTTGCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F8	260	MUPPITS	Doug	0.7232825	Y	SAMPLE	NASAL	Sample260
Sample261	TCGAGCCGATCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F9	261	MUPPITS	Doug	0.7466027	Y	SAMPLE	NASAL	Sample261
Sample262	CTCATCATGTTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F10	262	MUPPITS	Doug	0.7146580	Y	SAMPLE	NASAL	Sample262
Sample263	CCAGGGACTTCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F11	263	MUPPITS	Doug	0.4624980	Y	SAMPLE	NASAL	Sample263
Sample264	GCAATCCTTGCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	F12	264	MUPPITS	Doug	0.7218987	Y	SAMPLE	NASAL	Sample264
Sample265	CCTGCTTCCTTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G1	265	MUPPITS	Doug	0.6347577	Y	SAMPLE	NASAL	Sample265
Sample266	CAAGGCACAAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G2	266	MUPPITS	Doug	0.2237054	Y	SAMPLE	NASAL	Sample266
Sample267	GGCCTATAAGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G3	267	MUPPITS	Doug	0.7411266	Y	SAMPLE	NASAL	Sample267
Sample268	TCCATTTCATGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G4	268	MUPPITS	Doug	0.4955618	Y	SAMPLE	NASAL	Sample268
Sample269	TCGGCGATCATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G5	269	MUPPITS	Doug	0.2739442	Y	SAMPLE	NASAL	Sample269
Sample270	GTTTCACGCGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G6	270	MUPPITS	Doug	0.3904629	Y	SAMPLE	NASAL	Sample270
Sample271	ACAAGAACCTTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G7	271	MUPPITS	Doug	0.4172926	Y	SAMPLE	NASAL	Sample271
Sample272	TACTCTCTTAGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G8	272	MUPPITS	Doug	0.8186153	Y	SAMPLE	NASAL	Sample272
Sample273	AACTGTTCGCGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G9	273	MUPPITS	Doug	0.4832181	Y	SAMPLE	NASAL	Sample273
Sample274	CGAAGCATCTAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G10	274	MUPPITS	Doug	0.9070658	Y	SAMPLE	NASAL	Sample274
Sample275	GTTTGGCCACAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G11	275	MUPPITS	Doug	0.2904254	Y	SAMPLE	NASAL	Sample275
Sample276	TCAGGTTGCCCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	G12	276	MUPPITS	Doug	0.7737068	Y	SAMPLE	NASAL	Sample276
Sample277	TCATTCCACTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H1	277	MUPPITS	Doug	0.0353057	Y	SAMPLE	NASAL	Sample277
Sample278	GTCACATCACGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H2	278	MUPPITS	Doug	0.5271405	Y	SAMPLE	NASAL	Sample278
Sample279	CGACATTTCTCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H3	279	MUPPITS	Doug	0.4995205	Y	SAMPLE	NASAL	Sample279
Sample280	GGACGTTAACTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H4	280	MUPPITS	Doug	0.5273082	Y	SAMPLE	NASAL	Sample280
Sample281	TAGCAGTTGCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H5	281	MUPPITS	Doug	0.7975710	Y	SAMPLE	NASAL	Sample281
Sample282	CACGCTATTGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H6	282	MUPPITS	Doug	0.4739997	Y	SAMPLE	NASAL	Sample282
Sample283	AACTTCACTTCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H7	283	MUPPITS	Doug	0.5795815	Y	NTC	NTC	Sample283
Sample284	CCAGTGGATATA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H8	284	MUPPITS	Doug	0.3753977	Y	SAMPLE	NASAL	Sample284
Sample285	TGTGTGTAACGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H9	285	MUPPITS	Doug	0.2277067	Y	SAMPLE	NASAL	Sample285
Sample286	CCAATCGTGCAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H10	286	MUPPITS	Doug	0.5603315	Y	SAMPLE	NASAL	Sample286
Sample287	AGGCTAGCAGAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H11	287	MUPPITS	Doug	0.4723419	Y	SAMPLE	NASAL	Sample287
Sample288	GTCACTCCGAAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE3	H12	288	MUPPITS	Doug	0.9096698	Y	SAMPLE	NASAL	Sample288
Sample289	TACCGCTTCTTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A1	289	MUPPITS	Doug	0.8925276	Y	SAMPLE	NASAL	Sample289
Sample290	TGTGCGATAACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A2	290	MUPPITS	Doug	0.9573221	Y	SAMPLE	NASAL	Sample290
Sample291	GATTATCGACGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A3	291	MUPPITS	Doug	0.0347771	Y	SAMPLE	NASAL	Sample291
Sample292	GCCTAGCCCAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A4	292	MUPPITS	Doug	0.4335329	Y	SAMPLE	NASAL	Sample292
Sample293	GATGTATGTGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A5	293	MUPPITS	Doug	0.7829583	Y	SAMPLE	NASAL	Sample293
Sample294	ACTCCTTGTGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A6	294	MUPPITS	Doug	0.6411524	Y	SAMPLE	NASAL	Sample294
Sample295	GTCACGGACATT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A7	295	MUPPITS	Doug	0.4209977	Y	NTC	NTC	Sample295
Sample296	GCGAGCGAAGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A8	296	MUPPITS	Doug	0.3205170	Y	SAMPLE	NASAL	Sample296
Sample297	ATCTACCGAAGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A9	297	MUPPITS	Doug	0.5669690	Y	SAMPLE	NASAL	Sample297
Sample298	ACTTGGTGTAAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A10	298	MUPPITS	Doug	0.0917206	Y	SAMPLE	NASAL	Sample298
Sample299	TCTTGGAGGTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A11	299	MUPPITS	Doug	0.4511577	Y	SAMPLE	NASAL	Sample299
Sample300	TCACCTCCTTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	A12	300	MUPPITS	Doug	0.4536797	Y	SAMPLE	NASAL	Sample300
Sample301	GCACACCTGATA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B1	301	MUPPITS	Doug	0.8319708	Y	SAMPLE	NASAL	Sample301
Sample302	GCGACAATTACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B2	302	MUPPITS	Doug	0.7250419	Y	SAMPLE	NASAL	Sample302
Sample303	TCATGCTCCATT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B3	303	MUPPITS	Doug	0.9045506	Y	SAMPLE	NASAL	Sample303
Sample304	AGCTGTCAAGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B4	304	MUPPITS	Doug	0.8810210	Y	SAMPLE	NASAL	Sample304
Sample305	GAGAGCAACAGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B5	305	MUPPITS	Doug	0.5487953	Y	SAMPLE	NASAL	Sample305
Sample306	TACTCGGGAACT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B6	306	MUPPITS	Doug	0.3758758	Y	SAMPLE	NASAL	Sample306
Sample307	CGTGCTTAGGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B7	307	MUPPITS	Doug	0.4993556	Y	SAMPLE	NASAL	Sample307
Sample308	TACCGAAGGTAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B8	308	MUPPITS	Doug	0.4518669	Y	SAMPLE	NASAL	Sample308
Sample309	CACTCATCATTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B9	309	MUPPITS	Doug	0.8836449	Y	SAMPLE	NASAL	Sample309
Sample310	GTATTTCGGACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B10	310	MUPPITS	Doug	0.8679082	Y	SAMPLE	NASAL	Sample310
Sample311	TATCTATCCTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B11	311	MUPPITS	Doug	0.1071899	Y	SAMPLE	NASAL	Sample311
Sample312	TTGCCAAGAGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	B12	312	MUPPITS	Doug	0.3608415	Y	SAMPLE	NASAL	Sample312
Sample313	AGTAGCGGAAGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C1	313	MUPPITS	Doug	0.1400617	Y	NTC	NTC	Sample313
Sample314	GCAATTAGGTAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C2	314	MUPPITS	Doug	0.3059043	Y	SAMPLE	NASAL	Sample314
Sample315	CATACCGTGAGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C3	315	MUPPITS	Doug	0.4939922	Y	SAMPLE	NASAL	Sample315
Sample316	ATGTGTGTAGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C4	316	MUPPITS	Doug	0.5645008	Y	SAMPLE	NASAL	Sample316
Sample317	CCTGCGAAGTAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C5	317	MUPPITS	Doug	0.8507064	Y	SAMPLE	NASAL	Sample317
Sample318	TTCTCTCGACAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C6	318	MUPPITS	Doug	0.2763018	Y	SAMPLE	NASAL	Sample318
Sample319	GCTCTCCGTAGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C7	319	MUPPITS	Doug	0.4749681	Y	SAMPLE	NASAL	Sample319
Sample320	GTTAAGCTGACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C8	320	MUPPITS	Doug	0.0308518	Y	SAMPLE	NASAL	Sample320
Sample321	ATGCCATGCCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C9	321	MUPPITS	Doug	0.6346572	Y	SAMPLE	NASAL	Sample321
Sample322	GACATTGTCACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C10	322	MUPPITS	Doug	0.1139344	Y	SAMPLE	NASAL	Sample322
Sample323	GCCAACAACCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C11	323	MUPPITS	Doug	0.4960109	Y	SAMPLE	NASAL	Sample323
Sample324	ATCAGTACTAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	C12	324	MUPPITS	Doug	0.6291475	Y	SAMPLE	NASAL	Sample324
Sample325	TCCTCGAGCGAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D1	325	MUPPITS	Doug	0.2771668	Y	SAMPLE	NASAL	Sample325
Sample326	ACCCAAGCGTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D2	326	MUPPITS	Doug	0.5686787	Y	SAMPLE	NASAL	Sample326
Sample327	TGCAGCAAGATT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D3	327	MUPPITS	Doug	0.6011528	Y	SAMPLE	NASAL	Sample327
Sample328	AGCAACATTGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D4	328	MUPPITS	Doug	0.2828988	Y	SAMPLE	NASAL	Sample328
Sample329	GATGTGGTGTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D5	329	MUPPITS	Doug	0.6569169	Y	SAMPLE	NASAL	Sample329
Sample330	CAGAAATGTGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D6	330	MUPPITS	Doug	0.9222421	Y	SAMPLE	NASAL	Sample330
Sample331	GTAGAGGTAGAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D7	331	MUPPITS	Doug	0.6641881	Y	SAMPLE	NASAL	Sample331
Sample332	CGTGATCCGCTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D8	332	MUPPITS	Doug	0.6119982	Y	SAMPLE	NASAL	Sample332
Sample333	GGTTATTTGGCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D9	333	MUPPITS	Doug	0.8726254	Y	SAMPLE	NASAL	Sample333
Sample334	GGATCGTAATAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D10	334	MUPPITS	Doug	0.6452621	Y	SAMPLE	NASAL	Sample334
Sample335	GCATAGCATCAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D11	335	MUPPITS	Doug	0.9095719	Y	SAMPLE	NASAL	Sample335
Sample336	GTGTTAGATGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	D12	336	MUPPITS	Doug	0.3227007	Y	SAMPLE	NASAL	Sample336
Sample337	TTAGAGCCATGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E1	337	MUPPITS	Doug	0.9958060	Y	SAMPLE	NASAL	Sample337
Sample338	TGAACCCTATGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E2	338	MUPPITS	Doug	0.6155697	Y	SAMPLE	NASAL	Sample338
Sample339	AGAGTCTTGCCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E3	339	MUPPITS	Doug	0.6682389	Y	NTC	NTC	Sample339
Sample340	ACAACACTCCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E4	340	MUPPITS	Doug	0.1684485	Y	SAMPLE	NASAL	Sample340
Sample341	CGATGCTGTTGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E5	341	MUPPITS	Doug	0.7617579	Y	SAMPLE	NASAL	Sample341
Sample342	ACGACTGCATAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E6	342	MUPPITS	Doug	0.2591249	Y	SAMPLE	NASAL	Sample342
Sample343	ACGCGAACTAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E7	343	MUPPITS	Doug	0.6699329	Y	SAMPLE	NASAL	Sample343
Sample344	AGCTATGTATGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E8	344	MUPPITS	Doug	0.6674057	Y	SAMPLE	NASAL	Sample344
Sample345	ACGGGTCATCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E9	345	MUPPITS	Doug	0.5561233	Y	SAMPLE	NASAL	Sample345
Sample346	GAAACATCCCAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E10	346	MUPPITS	Doug	0.0725832	Y	SAMPLE	NASAL	Sample346
Sample347	CGTACTCTCGAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E11	347	MUPPITS	Doug	0.6160550	Y	SAMPLE	NASAL	Sample347
Sample348	TCAGTTCTCGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	E12	348	MUPPITS	Doug	0.9538218	Y	SAMPLE	NASAL	Sample348
Sample349	TCGTGCGTGTTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F1	349	MUPPITS	Doug	0.7362957	Y	SAMPLE	NASAL	Sample349
Sample350	GTTATCGCATGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F2	350	MUPPITS	Doug	0.5857433	Y	SAMPLE	NASAL	Sample350
Sample351	GATCACGAGAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F3	351	MUPPITS	Doug	0.8223391	Y	SAMPLE	NASAL	Sample351
Sample352	GTAAATTCAGGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F4	352	MUPPITS	Doug	0.8689296	Y	SAMPLE	NASAL	Sample352
Sample353	AGTGTTTCGGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F5	353	MUPPITS	Doug	0.9862476	Y	SAMPLE	NASAL	Sample353
Sample354	ACACGCGGTTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F6	354	MUPPITS	Doug	0.6486112	Y	SAMPLE	NASAL	Sample354
Sample355	TGGCAAATCTAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F7	355	MUPPITS	Doug	0.2026383	Y	SAMPLE	NASAL	Sample355
Sample356	CACCTTACCTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F8	356	MUPPITS	Doug	0.5163964	Y	SAMPLE	NASAL	Sample356
Sample357	TTAACCTTCCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F9	357	MUPPITS	Doug	0.6636819	Y	SAMPLE	NASAL	Sample357
Sample358	TGCCGTATGCCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F10	358	MUPPITS	Doug	0.0554755	Y	NTC	NTC	Sample358
Sample359	CGTGACAATAGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F11	359	MUPPITS	Doug	0.0207225	Y	SAMPLE	NASAL	Sample359
Sample360	CGCTACAACTCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	F12	360	MUPPITS	Doug	0.2281818	Y	SAMPLE	NASAL	Sample360
Sample361	TTAAGACAGTCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G1	361	MUPPITS	Doug	0.6897112	Y	SAMPLE	NASAL	Sample361
Sample362	TCTGCACTGAGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G2	362	MUPPITS	Doug	0.9929094	Y	SAMPLE	NASAL	Sample362
Sample363	CGCAGATTAGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G3	363	MUPPITS	Doug	0.8664908	Y	SAMPLE	NASAL	Sample363
Sample364	TGGGTCCCACAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G4	364	MUPPITS	Doug	0.9100804	Y	SAMPLE	NASAL	Sample364
Sample365	CACTGGTGCATA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G5	365	MUPPITS	Doug	0.5092845	Y	SAMPLE	NASAL	Sample365
Sample366	AACGTAGGCTCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G6	366	MUPPITS	Doug	0.2951709	Y	SAMPLE	NASAL	Sample366
Sample367	AGTTGTAGTCCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G7	367	MUPPITS	Doug	0.7967598	Y	SAMPLE	NASAL	Sample367
Sample368	TCGTCAAACCCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G8	368	MUPPITS	Doug	0.1968728	Y	SAMPLE	NASAL	Sample368
Sample369	TAATCGGTGCCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G9	369	MUPPITS	Doug	0.6676200	Y	SAMPLE	NASAL	Sample369
Sample370	TTGATCCGGTAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G10	370	MUPPITS	Doug	0.6591656	Y	SAMPLE	NASAL	Sample370
Sample371	CGGGTGTTTGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G11	371	MUPPITS	Doug	0.1870852	Y	SAMPLE	NASAL	Sample371
Sample372	TTGACCGCGGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	G12	372	MUPPITS	Doug	0.0991767	Y	SAMPLE	NASAL	Sample372
Sample373	GTGCAACCAATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H1	373	MUPPITS	Doug	0.2743989	Y	SAMPLE	NASAL	Sample373
Sample374	GCTTGAGCTTGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H2	374	MUPPITS	Doug	0.5649026	Y	SAMPLE	NASAL	Sample374
Sample375	CGCTGTGGATTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H3	375	MUPPITS	Doug	0.0472008	Y	SAMPLE	NASAL	Sample375
Sample376	CTGTCAGTGACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H4	376	MUPPITS	Doug	0.3955148	Y	SAMPLE	NASAL	Sample376
Sample377	ACGATTCGAGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H5	377	EMPTY	Doug	0.3885645	Y	EMPTY	EMPTY	Sample377
Sample378	GGTTCGGTCCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H6	378	EMPTY	Doug	0.9979978	Y	EMPTY	EMPTY	Sample378
Sample379	CTGATCCATCTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H7	379	DONOR	Doug	0.5401299	Y	SAMPLE	SALIVA	Sample379
Sample380	TATGTGCCGGCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H8	380	DONOR	Doug	0.8103156	Y	SAMPLE	SALIVA	Sample380
Sample381	TGGTCGCATCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H9	381	DONOR	Doug	0.1213106	Y	SAMPLE	SALIVA	Sample381
Sample382	TGTAAGACTTGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H10	382	EMPTY	Doug	0.6769711	Y	EMPTY	EMPTY	Sample382
Sample383	CGGATCTAGTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H11	383	EMPTY	Doug	0.4275346	Y	EMPTY	EMPTY	Sample383
Sample384	CGATCTTCGAGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE4	H12	384	EMPTY	Doug	0.9676501	Y	EMPTY	EMPTY	Sample384
Sample385	GTCGAATTTGCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A1	385	HALEY	Doug	0.9151426	Y	SAMPLE	STOOL	Sample385
Sample386	GCATCAGAGTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A2	386	HALEY	Doug	0.6922549	Y	SAMPLE	STOOL	Sample386
Sample387	GTGGTCATCGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A3	387	HALEY	Doug	0.5069642	Y	SAMPLE	STOOL	Sample387
Sample388	CTGAAGGGCGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A4	388	HALEY	Doug	0.5105577	Y	SAMPLE	STOOL	Sample388
Sample389	CGCTCACAGAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A5	389	HALEY	Doug	0.6578309	Y	SAMPLE	STOOL	Sample389
Sample390	ATTCGGTAGTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A6	390	HALEY	Doug	0.9484373	Y	SAMPLE	STOOL	Sample390
Sample391	CGAGCTGTTACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A7	391	HALEY	Doug	0.3869238	Y	SAMPLE	STOOL	Sample391
Sample392	CAACACATGCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A8	392	HALEY	Doug	0.4113079	Y	SAMPLE	STOOL	Sample392
Sample393	ATTCTCTCACGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A9	393	HALEY	Doug	0.0145308	Y	SAMPLE	STOOL	Sample393
Sample394	CGACTCTAAACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A10	394	HALEY	Doug	0.0032090	Y	SAMPLE	STOOL	Sample394
Sample395	GTCTTCAGCAAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A11	395	HALEY	Doug	0.5644562	Y	SAMPLE	STOOL	Sample395
Sample396	CGGATAACCTCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	A12	396	HALEY	Doug	0.3504660	Y	SAMPLE	STOOL	Sample396
Sample397	AGGGTGACTTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B1	397	HALEY	Doug	0.6770682	Y	SAMPLE	STOOL	Sample397
Sample398	GACTTCATGCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B2	398	HALEY	Doug	0.2397218	Y	SAMPLE	STOOL	Sample398
Sample399	GCCTGTCTGCAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B3	399	HALEY	Doug	0.5697592	Y	SAMPLE	STOOL	Sample399
Sample400	ACTGATGGCCTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B4	400	HALEY	Doug	0.5173269	Y	SAMPLE	STOOL	Sample400
Sample401	TTCGATGCCGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B5	401	HALEY	Doug	0.4764659	Y	SAMPLE	STOOL	Sample401
Sample402	TGTGGCTCGTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B6	402	HALEY	Doug	0.9955801	Y	SAMPLE	STOOL	Sample402
Sample403	AACTTTCAGGAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B7	403	HALEY	Doug	0.7839012	Y	NTC	NTC	Sample403
Sample404	TGCACGTGATAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B8	404	HALEY	Doug	0.9346177	Y	SAMPLE	STOOL	Sample404
Sample405	GTTCGGTGTCCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B9	405	HALEY	Doug	0.9748273	Y	SAMPLE	STOOL	Sample405
Sample406	AAGACAGCTATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B10	406	HALEY	Doug	0.9246091	Y	SAMPLE	STOOL	Sample406
Sample407	ATTGACCGGTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B11	407	HALEY	Doug	0.6805157	Y	SAMPLE	STOOL	Sample407
Sample408	TTCTCCATCACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	B12	408	HALEY	Doug	0.8238147	Y	SAMPLE	STOOL	Sample408
Sample409	CGTAGGTAGAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C1	409	HALEY	Doug	0.6371297	Y	SAMPLE	STOOL	Sample409
Sample410	ATTTAGGACGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C2	410	HALEY	Doug	0.0850860	Y	SAMPLE	STOOL	Sample410
Sample411	GGATAGCCAAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C3	411	HALEY	Doug	0.6701920	Y	SAMPLE	STOOL	Sample411
Sample412	TGGTTGGTTACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C4	412	HALEY	Doug	0.2637280	Y	SAMPLE	STOOL	Sample412
Sample413	GTCGTCCAAATG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C5	413	HALEY	Doug	0.2032890	Y	SAMPLE	STOOL	Sample413
Sample414	CAACGTGCTCCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C6	414	HALEY	Doug	0.1555723	Y	SAMPLE	STOOL	Sample414
Sample415	TACACAAGTCGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C7	415	HALEY	Doug	0.5058063	Y	SAMPLE	STOOL	Sample415
Sample416	GCGTCCATGAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C8	416	HALEY	Doug	0.8818387	Y	SAMPLE	STOOL	Sample416
Sample417	GTAATGCGTAAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C9	417	HALEY	Doug	0.9043368	Y	SAMPLE	STOOL	Sample417
Sample418	GTCGCCGTACAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C10	418	HALEY	Doug	0.8273181	Y	SAMPLE	STOOL	Sample418
Sample419	GGAATCCGATTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C11	419	HALEY	Doug	0.7795508	Y	SAMPLE	STOOL	Sample419
Sample420	CACCCGATGGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	C12	420	HALEY	Doug	0.0132335	Y	SAMPLE	STOOL	Sample420
Sample421	TTCTGAGAGGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D1	421	HALEY	Doug	0.0440445	Y	SAMPLE	STOOL	Sample421
Sample422	ATCCCTACGGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D2	422	HALEY	Doug	0.7512931	Y	SAMPLE	STOOL	Sample422
Sample423	GGTTCCATTAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D3	423	HALEY	Doug	0.8266436	Y	SAMPLE	STOOL	Sample423
Sample424	GTGTTCCCAGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D4	424	HALEY	Doug	0.7840277	Y	NTC	NTC	Sample424
Sample425	CCGAGGTATAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D5	425	CHERRY	Doug	0.7531060	Y	SAMPLE	STOOL	Sample425
Sample426	AGCGTAATTAGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D6	426	CHERRY	Doug	0.2184138	Y	SAMPLE	STOOL	Sample426
Sample427	CTCGTGAATGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D7	427	CHERRY	Doug	0.9636460	Y	SAMPLE	STOOL	Sample427
Sample428	AGGTGAGTTCTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D8	428	CHERRY	Doug	0.0846861	Y	SAMPLE	STOOL	Sample428
Sample429	CCTGTCCTATCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D9	429	CHERRY	Doug	0.7799461	Y	SAMPLE	STOOL	Sample429
Sample430	GGTTTAACACGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D10	430	CHERRY	Doug	0.4533962	Y	SAMPLE	STOOL	Sample430
Sample431	AGACAGTAGGAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D11	431	CHERRY	Doug	0.1432122	Y	SAMPLE	STOOL	Sample431
Sample432	GCCACGACTTAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	D12	432	CHERRY	Doug	0.0239207	Y	SAMPLE	STOOL	Sample432
Sample433	ATTGTTCCTACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E1	433	CHERRY	Doug	0.6489466	Y	SAMPLE	STOOL	Sample433
Sample434	GCCGTAAACTTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E2	434	CHERRY	Doug	0.4406283	Y	SAMPLE	STOOL	Sample434
Sample435	GCAGATTTCCAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E3	435	CHERRY	Doug	0.1471501	Y	SAMPLE	STOOL	Sample435
Sample436	AGATGATCAGTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E4	436	CHERRY	Doug	0.4089802	Y	SAMPLE	STOOL	Sample436
Sample437	GAGACGTGTTCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E5	437	CHERRY	Doug	0.5166969	Y	SAMPLE	STOOL	Sample437
Sample438	TATCACCGGCAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E6	438	CHERRY	Doug	0.8196788	Y	SAMPLE	STOOL	Sample438
Sample439	TATGCCAGAGAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E7	439	CHERRY	Doug	0.5337339	Y	SAMPLE	STOOL	Sample439
Sample440	AGGTCCAAATCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E8	440	CHERRY	Doug	0.8066327	Y	SAMPLE	STOOL	Sample440
Sample441	ACCGTGCTCACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E9	441	CHERRY	Doug	0.3695879	Y	SAMPLE	STOOL	Sample441
Sample442	CTCCCTTTGTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E10	442	CHERRY	Doug	0.4634412	Y	SAMPLE	STOOL	Sample442
Sample443	AGCTGCACCTAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E11	443	CHERRY	Doug	0.6159218	Y	SAMPLE	STOOL	Sample443
Sample444	CCTTGACCGATG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	E12	444	CHERRY	Doug	0.7459149	Y	SAMPLE	STOOL	Sample444
Sample445	CTATCATCCTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F1	445	CHERRY	Doug	0.5281308	Y	SAMPLE	STOOL	Sample445
Sample446	ACTCTAGCCGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F2	446	CHERRY	Doug	0.0532558	Y	SAMPLE	STOOL	Sample446
Sample447	CGATAGGCCTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F3	447	CHERRY	Doug	0.4322907	Y	SAMPLE	STOOL	Sample447
Sample448	AATGACCTCGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F4	448	CHERRY	Doug	0.1414017	Y	SAMPLE	STOOL	Sample448
Sample449	CTTAGGCATGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F5	449	CHERRY	Doug	0.2171243	Y	SAMPLE	STOOL	Sample449
Sample450	CCAGATATAGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F6	450	CHERRY	Doug	0.7982424	Y	SAMPLE	STOOL	Sample450
Sample451	GAGAGTCCACTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F7	451	CHERRY	Doug	0.0859159	Y	SAMPLE	STOOL	Sample451
Sample452	GAACGGGACGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F8	452	CHERRY	Doug	0.4391709	Y	SAMPLE	STOOL	Sample452
Sample453	ACGTGTAGGCTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F9	453	CHERRY	Doug	0.3332950	Y	NTC	NTC	Sample453
Sample454	GGTCTCCTACAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F10	454	CHERRY	Doug	0.6012575	Y	SAMPLE	STOOL	Sample454
Sample455	ACTGACTTAAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F11	455	CHERRY	Doug	0.7112106	Y	SAMPLE	STOOL	Sample455
Sample456	GATGCTGCCGTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	F12	456	CHERRY	Doug	0.3416685	Y	SAMPLE	STOOL	Sample456
Sample457	TTCCTAGGCCAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G1	457	CHERRY	Doug	0.0612829	Y	SAMPLE	STOOL	Sample457
Sample458	ATTAAGCCTGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G2	458	CHERRY	Doug	0.1821023	Y	SAMPLE	STOOL	Sample458
Sample459	TGGCTTTCTATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G3	459	CHERRY	Doug	0.8751809	Y	SAMPLE	STOOL	Sample459
Sample460	ACAGCTCAAACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G4	460	CHERRY	Doug	0.6658579	Y	SAMPLE	STOOL	Sample460
Sample461	GAGCGTATCCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G5	461	CHERRY	Doug	0.5145469	Y	SAMPLE	STOOL	Sample461
Sample462	ATGGGCGAATGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G6	462	CHERRY	Doug	0.1826208	Y	SAMPLE	STOOL	Sample462
Sample463	GATCTCTGGGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G7	463	CHERRY	Doug	0.5554142	Y	SAMPLE	STOOL	Sample463
Sample464	CATCATACGGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G8	464	CHERRY	Doug	0.8692514	Y	SAMPLE	STOOL	Sample464
Sample465	TACGGATTATGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G9	465	CHERRY	Doug	0.8906117	Y	SAMPLE	STOOL	Sample465
Sample466	ATAGCGAACTCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G10	466	CHERRY	Doug	0.5717385	Y	SAMPLE	STOOL	Sample466
Sample467	TAACGCTGTGTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G11	467	CHERRY	Doug	0.8688037	Y	SAMPLE	STOOL	Sample467
Sample468	AACCAAACTCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	G12	468	CHERRY	Doug	0.0271247	Y	SAMPLE	STOOL	Sample468
Sample469	GCCGTCTCGTAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H1	469	CHERRY	Doug	0.0321348	Y	NTC	NTC	Sample469
Sample470	CTGGGTATCTCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H2	470	CHERRY	Doug	0.1922356	Y	SAMPLE	STOOL	Sample470
Sample471	GACTACCCGTTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H3	471	CHERRY	Doug	0.0614275	Y	SAMPLE	STOOL	Sample471
Sample472	GCGTTGCAAACT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H4	472	CHERRY	Doug	0.0367892	Y	SAMPLE	STOOL	Sample472
Sample473	AACCGCATAAGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H5	473	CHERRY	Doug	0.3871574	Y	SAMPLE	STOOL	Sample473
Sample474	ACCTTACACCTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H6	474	HALEY	Doug	0.3899782	Y	SAMPLE	STOOL	Sample474
Sample475	GTAGGTGCTTAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H7	475	HALEY	Doug	0.3126317	Y	SAMPLE	STOOL	Sample475
Sample476	CGCATTTGGATG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H8	476	HALEY	Doug	0.2483845	Y	SAMPLE	STOOL	Sample476
Sample477	ATAACATGTGCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H9	477	HALEY	Doug	0.7311695	Y	SAMPLE	STOOL	Sample477
Sample478	CTTGAGAAATCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H10	478	HALEY	Doug	0.5477358	Y	SAMPLE	STOOL	Sample478
Sample479	CTACACAGCACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H11	479	DONOR	Doug	0.1998826	Y	SAMPLE	STOOL	Sample479
Sample480	GAAATGCTACGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE5	H12	480	DONOR	Doug	0.6671454	Y	SAMPLE	STOOL	Sample480
Sample481	CACCGAAATCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A1	481	RYUTARO	Doug	0.6570242	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample481
Sample482	TGACGTAGAACT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A2	482	RYUTARO	Doug	0.5935104	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample482
Sample483	CTATGCCGGCTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A3	483	RYUTARO	Doug	0.2999305	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample483
Sample484	GTGGTATGGGAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A4	484	RYUTARO	Doug	0.8122738	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample484
Sample485	TGTACCAACCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A5	485	RYUTARO	Doug	0.0795235	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample485
Sample486	AGGGTACAGGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A6	486	RYUTARO	Doug	0.8734735	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample486
Sample487	AGAGTGCTAATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A7	487	RYUTARO	Doug	0.7096850	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample487
Sample488	TTGGCGGGTTAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A8	488	RYUTARO	Doug	0.0217844	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample488
Sample489	CACGATGGTCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A9	489	RYUTARO	Doug	0.9641195	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample489
Sample490	GTCACCAATCCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A10	490	RYUTARO	Doug	0.7057182	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample490
Sample491	CACTAACAAACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A11	491	RYUTARO	Doug	0.7692963	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample491
Sample492	TTCCAGGCAGAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	A12	492	RYUTARO	Doug	0.9698262	Y	SAMPLE	DNA (MOUSE ORAL SWAB)	Sample492
Sample493	TATGGTACCCAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B1	493	EMPTY	Doug	0.2318000	N	EMPTY	EMPTY	Sample493
Sample494	CACGACTTGACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B2	494	EMPTY	Doug	0.3458918	N	EMPTY	EMPTY	Sample494
Sample495	CTTGGAGGCTTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B3	495	EMPTY	Doug	0.1490522	N	EMPTY	EMPTY	Sample495
Sample496	ACGTGGTTCCAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B4	496	EMPTY	Doug	0.5550656	N	EMPTY	EMPTY	Sample496
Sample497	GACGCTTTGCTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B5	497	EMPTY	Doug	0.8365000	N	EMPTY	EMPTY	Sample497
Sample498	ACAGGGTTTGTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B6	498	EMPTY	Doug	0.1663526	N	EMPTY	EMPTY	Sample498
Sample499	GCCTATGAGATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B7	499	EMPTY	Doug	0.1749379	N	EMPTY	EMPTY	Sample499
Sample500	CAAACCTATGGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B8	500	EMPTY	Doug	0.9650901	N	EMPTY	EMPTY	Sample500
Sample501	ATCGCTTAAGGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B9	501	EMPTY	Doug	0.2603262	N	EMPTY	EMPTY	Sample501
Sample502	ACCATCCAACGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B10	502	EMPTY	Doug	0.4161385	N	EMPTY	EMPTY	Sample502
Sample503	GCAATAGGAGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B11	503	EMPTY	Doug	0.9815977	N	EMPTY	EMPTY	Sample503
Sample504	CCGAACGTCACT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	B12	504	EMPTY	Doug	0.7947097	N	EMPTY	EMPTY	Sample504
Sample505	ACACCAACACCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C1	505	EMPTY	Doug	0.2716651	N	EMPTY	EMPTY	Sample505
Sample506	CCATCACATAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C2	506	EMPTY	Doug	0.9744303	N	EMPTY	EMPTY	Sample506
Sample507	CGACACGGAGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C3	507	EMPTY	Doug	0.7880179	N	EMPTY	EMPTY	Sample507
Sample508	GAACCTATGACA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C4	508	EMPTY	Doug	0.5155968	N	EMPTY	EMPTY	Sample508
Sample509	ATGCCGGTAATA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C5	509	EMPTY	Doug	0.6356287	N	EMPTY	EMPTY	Sample509
Sample510	GAACAGCTCTAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C6	510	EMPTY	Doug	0.8841456	N	EMPTY	EMPTY	Sample510
Sample511	GTGAGTCATACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C7	511	EMPTY	Doug	0.6304104	N	EMPTY	EMPTY	Sample511
Sample512	TGGCCGTTACTG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C8	512	EMPTY	Doug	0.8371140	N	EMPTY	EMPTY	Sample512
Sample513	TAGAGCTGCCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C9	513	EMPTY	Doug	0.4307193	N	EMPTY	EMPTY	Sample513
Sample514	ATCTAGTGGCAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C10	514	EMPTY	Doug	0.2263067	N	EMPTY	EMPTY	Sample514
Sample515	CCTTCAATGGGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C11	515	EMPTY	Doug	0.1955347	N	EMPTY	EMPTY	Sample515
Sample516	TTGACGACATCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	C12	516	EMPTY	Doug	0.4042136	N	EMPTY	EMPTY	Sample516
Sample517	ACATACTGAGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D1	517	EMPTY	Doug	0.0983255	N	EMPTY	EMPTY	Sample517
Sample518	GGCTAAACTATG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D2	518	EMPTY	Doug	0.2017935	N	EMPTY	EMPTY	Sample518
Sample519	AAGAGCAGAGCC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D3	519	EMPTY	Doug	0.8587315	N	EMPTY	EMPTY	Sample519
Sample520	GGAGAGATCACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D4	520	EMPTY	Doug	0.0872250	N	EMPTY	EMPTY	Sample520
Sample521	TCAACCCGTGAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D5	521	EMPTY	Doug	0.6015601	N	EMPTY	EMPTY	Sample521
Sample522	GTTTGAAACACG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D6	522	EMPTY	Doug	0.2887290	N	EMPTY	EMPTY	Sample522
Sample523	AGAGAGACAGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D7	523	EMPTY	Doug	0.7298209	N	EMPTY	EMPTY	Sample523
Sample524	TCGCCAGTGCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D8	524	EMPTY	Doug	0.3950536	N	EMPTY	EMPTY	Sample524
Sample525	GCTCAGGACTCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D9	525	EMPTY	Doug	0.1246456	N	EMPTY	EMPTY	Sample525
Sample526	CACTTTGGGTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D10	526	EMPTY	Doug	0.7246429	N	EMPTY	EMPTY	Sample526
Sample527	TCTAGCCTGGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D11	527	EMPTY	Doug	0.8396658	N	EMPTY	EMPTY	Sample527
Sample528	AATGCAATGCGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	D12	528	EMPTY	Doug	0.3747440	N	EMPTY	EMPTY	Sample528
Sample529	CGAATGAGTCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E1	529	EMPTY	Doug	0.7752272	N	EMPTY	EMPTY	Sample529
Sample530	CAACGCTAGAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E2	530	EMPTY	Doug	0.5169678	N	EMPTY	EMPTY	Sample530
Sample531	ATCAGAGCCCAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E3	531	EMPTY	Doug	0.3010501	N	EMPTY	EMPTY	Sample531
Sample532	TCTGTAGAGCCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E4	532	EMPTY	Doug	0.0714407	N	EMPTY	EMPTY	Sample532
Sample533	CCGACTCTAGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E5	533	EMPTY	Doug	0.0593549	N	EMPTY	EMPTY	Sample533
Sample534	ATCCTACGAGCA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E6	534	EMPTY	Doug	0.4211960	N	EMPTY	EMPTY	Sample534
Sample535	GACAACGAATCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E7	535	EMPTY	Doug	0.4271089	N	EMPTY	EMPTY	Sample535
Sample536	TGCGGTTGACTC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E8	536	EMPTY	Doug	0.1233769	N	EMPTY	EMPTY	Sample536
Sample537	TGAGAAGAAAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E9	537	EMPTY	Doug	0.5751844	N	EMPTY	EMPTY	Sample537
Sample538	TCGGATCTGTGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E10	538	EMPTY	Doug	0.2000258	N	EMPTY	EMPTY	Sample538
Sample539	GCCGGTACTCTA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E11	539	EMPTY	Doug	0.2574557	N	EMPTY	EMPTY	Sample539
Sample540	CACAGGATTACC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	E12	540	EMPTY	Doug	0.3747692	N	EMPTY	EMPTY	Sample540
Sample541	CGATATCAGTAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F1	541	EMPTY	Doug	0.4664447	N	EMPTY	EMPTY	Sample541
Sample542	CATAAGGGAGGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F2	542	EMPTY	Doug	0.5529594	N	EMPTY	EMPTY	Sample542
Sample543	TGTGTTACTCCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F3	543	EMPTY	Doug	0.6922730	N	EMPTY	EMPTY	Sample543
Sample544	GGTACCTGCAAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F4	544	EMPTY	Doug	0.4640710	N	EMPTY	EMPTY	Sample544
Sample545	TCGCCTATAAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F5	545	EMPTY	Doug	0.8224955	N	EMPTY	EMPTY	Sample545
Sample546	AGTGGCACTATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F6	546	EMPTY	Doug	0.9239143	N	EMPTY	EMPTY	Sample546
Sample547	TAACCCGATAGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F7	547	EMPTY	Doug	0.2051176	N	EMPTY	EMPTY	Sample547
Sample548	GTGTGCTAACGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F8	548	EMPTY	Doug	0.9548578	N	EMPTY	EMPTY	Sample548
Sample549	CTTGCGGCAATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F9	549	EMPTY	Doug	0.9131553	N	EMPTY	EMPTY	Sample549
Sample550	TGAGGTTTGATG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F10	550	EMPTY	Doug	0.6630253	N	EMPTY	EMPTY	Sample550
Sample551	ATTGCTGGTCGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F11	551	EMPTY	Doug	0.4528600	N	EMPTY	EMPTY	Sample551
Sample552	AAGAAGCCGGAC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	F12	552	EMPTY	Doug	0.0859553	N	EMPTY	EMPTY	Sample552
Sample553	ACGGGATACAGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G1	553	EMPTY	Doug	0.6442946	N	EMPTY	EMPTY	Sample553
Sample554	AAGAGTCTCTAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G2	554	EMPTY	Doug	0.3287575	N	EMPTY	EMPTY	Sample554
Sample555	TCCGTCATGGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G3	555	EMPTY	Doug	0.2569299	N	EMPTY	EMPTY	Sample555
Sample556	AGATCTATGCAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G4	556	EMPTY	Doug	0.5927613	N	EMPTY	EMPTY	Sample556
Sample557	GCACAAGGCAAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G5	557	EMPTY	Doug	0.6158518	N	EMPTY	EMPTY	Sample557
Sample558	CGGCAAACACTT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G6	558	EMPTY	Doug	0.9140748	N	EMPTY	EMPTY	Sample558
Sample559	GCGAGTTCCTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G7	559	EMPTY	Doug	0.9235337	N	EMPTY	EMPTY	Sample559
Sample560	TTCCGAATCGGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G8	560	EMPTY	Doug	0.3167997	N	EMPTY	EMPTY	Sample560
Sample561	TACCTAGTGAGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G9	561	EMPTY	Doug	0.7211587	N	EMPTY	EMPTY	Sample561
Sample562	CGTTCTGGTGGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G10	562	EMPTY	Doug	0.8592354	N	EMPTY	EMPTY	Sample562
Sample563	TTGGTCTCCTCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G11	563	EMPTY	Doug	0.6483799	N	EMPTY	EMPTY	Sample563
Sample564	CTGCATACTGAG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	G12	564	EMPTY	Doug	0.7191162	N	EMPTY	EMPTY	Sample564
Sample565	CAGGGCCTTTGT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H1	565	EMPTY	Doug	0.2333210	N	EMPTY	EMPTY	Sample565
Sample566	CGATGAATATCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H2	566	EMPTY	Doug	0.9342568	N	EMPTY	EMPTY	Sample566
Sample567	GTCAATTAGTGG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H3	567	EMPTY	Doug	0.7022118	N	EMPTY	EMPTY	Sample567
Sample568	AGTACGCAGTCT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H4	568	EMPTY	Doug	0.7451738	N	EMPTY	EMPTY	Sample568
Sample569	AGCAGCTATTGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H5	569	EMPTY	Doug	0.7244651	N	EMPTY	EMPTY	Sample569
Sample570	CTCGGATAGATC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H6	570	EMPTY	Doug	0.7923722	N	EMPTY	EMPTY	Sample570
Sample571	TTCCCGAAACGA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H7	571	EMPTY	Doug	0.4131243	N	EMPTY	EMPTY	Sample571
Sample572	GAACTTTAGCGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H8	572	EMPTY	Doug	0.0780754	N	EMPTY	EMPTY	Sample572
Sample573	TCCTTAGAAGGC	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H9	573	EMPTY	Doug	0.9269562	N	EMPTY	EMPTY	Sample573
Sample574	GATGGACTTCAA	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H10	574	EMPTY	Doug	0.4581629	N	EMPTY	EMPTY	Sample574
Sample575	TACTGAGCCTCG	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H11	575	EMPTY	Doug	0.5805108	N	EMPTY	EMPTY	Sample575
Sample576	AGAAGGCCTTAT	GTGTGCCAGCMGCCGCGGTAA	GTGTGCCAGCMGCCGCGGTAA	PLATE6	H12	576	EMPTY	Doug	0.0159409	N	EMPTY	EMPTY	Sample576

Processing a Raw NextSeq Run into an ASV table (QIIME)

Before you begin, if you have a SampleSheet.csv file in the raw run files, delete it! Katie has encountered unexpected bugs in the past due to this file.

The steps needed to process a NextSeq run and develop an ASV table have already been pipelined. Assuming the runs have been set up as above (run directory in /NextSeq_data/ and mapping file in /NextSeq_Processed/mapping_file/ with the modified formatting), you are ready to get started. Katie has recently upgraded the pipeline to utilize a Docker container so there is no need to install any packages to run the pipeline now. You can literally just push “go!”!

Once complete, start processing the run:

cd /wynton/group/lynch/NextSeq_Processed/
qsub -M {your-email} scripts/qiime_mapping_file_pipeline.sh 190826_NS500170_0076_AH772CBGXC Nextseq_190826_mapping.txt

The -M option will send you an e-mail when the process finishes. scripts/complete_16s_pipeline.sh is the name of the bash file with all of the commands. 190826_NS500170_0076_AH772CBGXC is the run name. Nextseq_190826_mapping.txt is the mapping file (which should live in the mapping_files directory). After several hours (16-24), you will have the following directories in the directory for this run within NextSeq_Processed:

submission: This directory contains all of your R1 and R2 files separated by sample ID. This is what gets uploaded to SRA and also what is used for DADA2.
FASTQC contains the results from FastQC You will need to download the files onto your computer to be able to view them. Katie has also implemented MultiQC which gives an overview of the FastQC results as one output file instead of a large set of FastQC files and going through them individually.
dada2_output contains all of the files generated from DADA2. These include:
- TrackedReadsThruDADA2.csv
- TaxonomyBootstraps.csv
- {runname}_seqs.txt (Just the ASVs)
- {runname}_tax_table.txt (Taxonomy Table from DADA2)
- {runname}_tax_table_DECIPHER.txt (Taxonomy Table from DECIPHER)
- {runname}_otutable.txt (ASV Table of counts)
- dada2_result_object.RData (Final dada2 object – if you need to troubleshoot something, you can load this object back into your environment)
- dada2_phy_obj_raw.rds (Raw phyloseq object from the DADA2 script)
- dada2_optim_tree.tre (Tree built from sequences)
- dada2_phy_pruned_wtree.rds (Phyloseq object with low-prevalence filtering and a tree object)
- Combined_Cleaning_Figure.pdf (Figure generated before and after negative control filtering – definitely review this figure to confirm that the defaults worked for your data)
- phyloseq_noneg.rds (Final phyloseq object with negative control signal removed as per the figure)

The file that you will use for subsequent analysis is phyloseq_noneg.rds. If you don’t get some of the later files, you may need to start with dada2_phy_obj_raw.rds and work through the interim steps separately.

Steps in the Script

bcl2fastq

Illumina’s proprietary hyper-compressed files are called BCL (or base call) files. They can be easily transformed into usable FastQ (Q because the quality information is included) files using this command. We’re not providing a mapping file, so all obtained reads are placed into “Undetermined” files, which means that the script wasn’t able to associate a barcode with a sample name. For this pipeline, this is fine and expected.

Undetermined FastQC

Next, we perform FastQC on the large undetermined files, which means that we are getting an overall quality profile of the reads. This gives us an additional sense if anything went awry with the run.

Split Libraries

“Splitting libraries” is essentially the practice of assigning a sample name to a read, and this is done with your well-formatted mapping file via some QIIME scripts. It matches the barcode from the FastQ file of barcodes to the list of barcodes in your mapping file and creates a new “pool” of sequences that have been assigned to a sample.

Poly-G Filter

The NextSeq in particular is on a two-color system. That means that by using just red and green colors, it can relay information for all four nucleotides. How, you ask? Through permutation! In other words, only green and only red are two nucleotides, but you get the other two by doing both and neither, respectively! How cool!!! Except…. Gs are created when you get no light, and sometimes you can get no light for reasons beyond a G nucleotide, creating long stretches of Gs in your sequence data. This makes DADA2 unhappy, so I use bbduk to filter out these “poly G” reads using an entropy (or sequence similarity score) of 0.2.

Make Sample-Specific FASTQs

Pretty straight-forward. Just makes one fastq file for each of the forward and reverse reads for each sample, which is the input to DADA2’s algorithm.

Pipeline #2 (Illumina Sample Sheet/Version 2)

As of recently, a pipeline based on the formatting of the Illumina Sample Sheet has been developed. This is likely the script that you will be using most-frequently unless you are working with a “legacy” run. The components of this pipeline are essentially the same as described above with a few behind-the-scenes modifications that allow the use of Illumina’s bcl2fastq software to actually perform the “splitting libraries” step instead of having QIIME do it (and raising reviewer eyes when trying to write methods).

Rules for the Illumina Sample Sheet

The rules associated with the Illumina Sample Sheet are annoyingly different, but relatively simple compared to the QIIME sample sheet. Specifically:

Sample names can only contain ‘-’ and ’_’ as non-alphanumeric characters. Which means no periods, slashes, etc.
Again, no empty values anywhere in the dataset
The Illumina Sample Sheet should be saved as a CSV (and not Excel’s “top” CSV format with UTF-8 encoding, but the one lower down: Comma Separated Values (.csv)). This is annoying but vital.
Sample sheets should be named similarly to how we’ve named them in QIIME format, except that the extension will be .csv instead of .txt. So, for example, the Illumina sheet should be named: Nextseq_YYMMDD_mapping.csv

Using the new script

After uploading the mapping file to /wynton/group/lynch/NextSeq_Processed/mapping_files/, log in to Wynton. Again, all software and R packages you need to run the pipeline are now “pre-installed” via a Docker container, so you can simply initiate the script by running:

cd /wynton/group/lynch/NextSeq_Processed/
qsub scripts/illumina_sample_sheet_pipeline.sh 190826_NS500170_0076_AH772CBGXC Nextseq_190826_mapping.csv

Post-Processing Steps

Low-prevalence filtering and negative control signal filtering are both part of our “usual” processing and filtering methods for most datasets and are completed automatically as part of the script above. Multiply-rarefying, however, is a process that requires a few additional steps to identify the optimal depth, so it needs to be done manually.

1. Low-prevalence filtering

Next, we want to remove any SVs in our table that may be contaminants, or generally present in extremely low counts relative to our total count, cutting down on noise in our data. This is typically 0.0001% of the total read count in your dataset.

2. Negative control filtering

Next, we use a script to remove signal deriving from the negative controls from our samples. This form of negative control filtering outright removes any SVs found in more than 15% of your negative controls and less than 15% of samples. Among the SVs that remaine, the average read count within negative controls is subtracted from the read count in samples, and negative numbers are returned to 0.

The script then returns a NTC-filtered phyloseq object. Also output by the script is a figure showing the distribution of taxa before and after running the script (Combined_Cleaning_Figure.pdf). An example output is shown here (this plot coming from running the same example SV table through the NTC script):

Image

We can tell from this figure that taxa like Pseudomonas and Delftia were removed, and while some taxa also present in negative controls remain, they are very likely to be biologically meaningful. Further examination of this data (not shown) found that remaining taxa had a very low total read count within the negative controls. Hence, the lack of movement of those taxa.

Normalizing for Differences in Read Depth

All microbiome data needs to be normalized for differences in read depth. This can be done through either Multiply Rarefying or Variance Stabilized Transformations.

Considerations for your own dataset should be driven by the questions you wish to answer. Our preference is for multiply-rarefying, but if you have low-abundance samples that you cannot filter out you may want to consider variance-stabilized transformations.

A. Rarefying

Rarefying means to sub-sample each sample to an even read depth. We use a method for representative rarefaction in which we sub-sample each sample 100 times and choose the randomized sample at the center of a distance matrix. However, before we can carry out this process, we need to determine the optimal depth. This depth is that at which there is no additional diversity to gain and you retain an optimal number of samples (samples with a read depth lower than the rarefying depth are removed).

I have built a script to carry out this process. In order to use it, you can run:

Rscript /wynton/group/lynch/kmccauley/dada2_files/alpha_rarefaction.R phyloseq_noneg.rds transpose

The transpose at the end of the script indicates that the table of counts should be transposed before generating rarefaction curves. If you wind up with a figure that shows rarefaction curves for sequence names, change the transpose to another value (all that matters is that it’s not transpose but is still one word).

This script runs slowly. Once it’s done, you will get two outputs from this command (1) a table of read depths and the number of samples remaining at that read depth and (2) a figure representing the curves for each sample. In order to view a figure made on Wynton, you can copy it to your desktop using either your favorite FTP or the following scp command from your computer:

scp {username}@dt2.wynton.ucsf.edu:{image_location}/Alpha_Rarefaction_Curve.pdf ~/Downloads/

Image

From this figure and the associated table printed in your console, you want to identify the depth where diversity plateaus, but you don’t end up losing many samples. In this example, we would likely pick something in the 50,000-70,000 read range, since diversity has plateaued and we start losing samples due to read depth soon after.

To initiate the multiply-rarefying step, you can run the following script from the location of your phyloseq object on Wynton. Before this point, you likely want to copy the phyloseq_noneg.rds file into your own analysis directory, perhaps giving it a more illustrative name.

Rscript /wynton/group/lynch/kmccauley/dada2_files/Multiply_Rarefy_phy.R phyloseq_noneg.rds 50000 phyloseq_mrare.rds

The first object is the name/location of the script. phyloseq_noneg.rds indicates the name of the input phyloseq object; 50000 is the planned read depth; phyloseq_mrare.rds is the name of the output object. If you’d rather not specify an output name, one will be chosen for you.

Procrustes

Let’s say you’re curious to know if the community composition of a dataset rarefied down to 50,000 reads is more or less informative than one rarefied to 70,000 reads. You can rarefy at both depths and then use a procrustes test to determine if their structure is significantly different, and I have developed a script to test this. To do this, you can run something like the following:

## Rarefy to 50k reads
Rscript /wynton/group/lynch/kmccauley/dada2_files/Multiply_Rarefy_phy.R phyloseq_noneg.rds 50000 phyloseq_mrare_50k.rds
## Rarefy to 70k reads
Rscript /wynton/group/lynch/kmccauley/dada2_files/Multiply_Rarefy_phy.R phyloseq_noneg.rds 70000 phyloseq_mrare_70k.rds
## Compare with Procrustes
Rscript /wynton/group/lynch/kmccauley/dada2_files/procrustes.R phyloseq_mrare_50k.rds phyloseq_mrare_70k.rds

The fun thing about the output is that if there is a significant P-value, it means that there is not a significant difference between the two datasets. The default distance matrix is currently Bray Curtis, but you can specify your preferred distance matrix by using one of the phyloseq distance matrix names at the end of the function (bray, canberra, unifrac, wunifrac).

B. Variance Stabilized Tranformations

We can also use the variance stabilized transformation, which can be applied before downstream analyses. Despite allowing for small read depths, you’ll still want to consider removing samples with less than 1000 reads since those profiles may not be as robust. However, if you are working with ultra-low-burden samples you may not be able to do that either. As we start getting into some of these circumstances, a chat with Katie or Sue may be helpful in determining how best to move forward given your study.

library(DESeq2)
library(phyloseq)
## read in the saved RDS file:
phy <- readRDS("phyloseq_noneg.rds")
phy.filt <- subset_samples(phy, sample_sums(phy) > 1000) ## filter to samples with greater than 1000 reads
phy.vst <- phy.filt ## Initialize a new object where we'll save the VST object / keeps us from overwriting the original data.
deseqdat <- phyloseq_to_deseq2(phy.vst, ~1) ## We can use this ~1 as our "design formula" since we're looking to normalize the data.
deseqdat2 = estimateSizeFactors(deseqdat, type="poscounts")
otu_table(phy.vst) <- otu_table(counts(deseqdat2, normalized=TRUE),taxa_are_rows=TRUE) ## Update OTU table in the VST object we saved.

At this point, you’re now ready to start the fun part! Analyzing and visualizing your data!

Acknowlegements

Katie would like to give many thanks to Danny Li who helped develop the initial version of the analysis pipeline, as well as the several members of the Lynch Lab and BCMM who provided invaluable constructive feedback along the way.

16s Processing Methods

Katie McCauley (kathryn.mccauley@ucsf.edu)

Last Modified: October 2022 (v2.1)